Collagen from fish scales serves to lower the risk of consuming collagen from other sources while also reducing land pollution. The scales of the sardine and emperor fish breeds were coughed up heavily and had more number of scales. The pretreatment, hydrolysis, and extraction procedure ratios applied to the scales of two different fish breeds varied according to the water level and temperature. The microstructure, elements, and functional groups were analyzed using SEM and FTIR. The results of the SEM and FTIR analysis demonstrated the most suitable collagen powder extraction ratio. The purest collagen was produced using 5 grams of emperor fish scales, 0.1 N NaOH for 3 days of pretreatment, 0.5 M HCl for 3 days of hydrolyzation, and 50 ml of double-distilled water utilized at 80°C. Additionally, this study demonstrated that collagen may be obtained by the drying process at room temperature. Comparing this procedure to freeze drying, it is less expensive. Moreover, this isolation was done by without using NaCl.